Development of collagen peptide raw materials from milkfish scale waste (Chanos Chanos) as a bioactive emulsifier
Keywords:
milkfish scales, collagen, emulsifying agentAbstract
Collagen peptides produced by milkfish (Chanos chanos) scales can be an alternative to replace collagen from mammals. Besides the high price of mammalian collagen, it also risks spreading infectious diseases like Bovine Spongiform Encephalopathy (BSE) and Transmissible spongiform encephalopathies (TSEs). This study aims to identify the effect of acetic acid and pepsin concentrations in optimizing the extraction of collagen peptides from milkfish scales due to waste and their application as a bioactive emulsifier. This study used an experimental design using a completely randomized design (CRD) with a 3 x 2 factorial pattern with three replications. The first factor is three levels of acetic acid concentration, and the second is three levels of pepsin concentration. The results of this study on the proximate analysis of collagen peptides showed that the results of the moisture content test, ash content test, protein content test, fat content test, and pH test met the requirements for collagen quality.
Downloads
References
Alhana, A., Suptijah, P., & Tarman, K. (2015). Extraction and characterization of collagen from sea cucumber flesh. Jurnal Pengolahan Hasil Perikanan Indonesia, 18(2).
Anwar, H.S., Antasari, M., Hasni, D., Sapriani, N., Rohaya, S., & Winarti, C. (2017). Kombinasi Pati Sukun Termodifikasi Osa (Octenyl Succinic Anhydride) Dan Lesitin Sebagai Penstabil Emulsi Minyak Dalam Air. Jurnal Penelitian Pascapanen Pertanian, 14(3), 124 - 133.
AOAC. (2005). Official Methods of Analysis. Washington: Association of Official Analytical Chemists.
Astawan, M., & Aviana, T. (2003). The Influence of Solvent Variety and Drying Method on Physical, Chemical and Functional Characteristic of Shark Skin Gelatin. Jurnal Teknologi dan Industri Pangan, 14(1), 7-7.
Aulton, M. E., & Cooper, J. W. (2002). Pharmaceutics: The science of dosage form design. (No Title).
Bhati, R., & Nagrajan, R. K. (2012). A detailed review on oral mucosal drug delivery system. Int J Pharm Sci Res, 3(3), 659.
Cardoso, C., Kingdon, D., & Ellenbogen, M. A. (2014). A meta-analytic review of the impact of intranasal oxytocin administration on cortisol concentrations during laboratory tasks: moderation by method and mental health. Psychoneuroendocrinology, 49, 161-170. https://doi.org/10.1016/j.psyneuen.2014.07.014
Chuaychan, S., Benjakul, S., & Kishimura, H. (2015). Characteristics of acid-and pepsin-soluble collagens from scale of seabass (Lates calcarifer). LWT-Food Science and Technology, 63(1), 71-76. https://doi.org/10.1016/j.lwt.2015.03.002
Depkes RI, 1986, Jenis Jenis Ekstraksi, Departemen Kesehatan RI, Jakarta.
Dirjen Perikanan Budidaya KKP RI. 2012. Panduan Penilaian Sertifikasi Cara Budidaya Ikan yang Baik (CBIB). Jakarta
Doki?, L., Krstonoši?, V., & Nikoli?, I. (2012). Physicochemical characteristics and stability of oil-in-water emulsions stabilized by OSA starch. Food Hydrocolloids, 29(1), 185-192. https://doi.org/10.1016/j.foodhyd.2012.02.008
Dybowska, B. E. (2011). Whey protein-stabilized emulsion properties in relation to thermal modification of the continuous phase. Journal of Food Engineering, 104(1), 81-88. https://doi.org/10.1016/j.jfoodeng.2010.11.030
Ennaas, N., Hammami, R., Gomaa, A., Bédard, F., Biron, É., Subirade, M., ... & Fliss, I. (2016). Collagencin, an antibacterial peptide from fish collagen: Activity, structure and interaction dynamics with membrane. Biochemical and biophysical research communications, 473(2), 642-647. https://doi.org/10.1016/j.bbrc.2016.03.121
Ermawati, D. E., Martodihardjo, S., & Sulaiman, T. S. (2017). Optimasi komposisi emulgator formula emulsi air dalam minyak jus buah stroberi (Fragaria vesca L.) dengan metode simplex lattice design. Journal of Pharmaceutical Science and Clinical Research, 2(2), 78-89.
Hartayanie, L., & Adriani, M. (2014). Karakteristik Emulsi Santan Dan Minyak Kedelai Yang Ditambah Gum Arab Dan Sukrosa Ester. Journal of Food Technology & Industry/Jurnal Teknologi & Industri Pangan, 25(2), 152.
Jafari, H., Lista, A., Siekapen, M. M., Ghaffari-Bohlouli, P., Nie, L., Alimoradi, H., & Shavandi, A. (2020). Fish collagen: extraction, characterization, and applications for biomaterials engineering. Polymers, 12(10), 2230.
Jamilah, B., Hartina, M. U., Hashim, D. M., & Sazili, A. Q. (2013). Properties of collagen from barramundi (Lates calcarifer) skin. International food research journal, 20(2), 835.
Ketnawa, S., Martínez-Alvarez, O., Benjakul, S., & Rawdkuen, S. (2016). Gelatin hydrolysates from farmed Giant catfish skin using alkaline proteases and its antioxidative function of simulated gastro-intestinal digestion. Food Chemistry, 192, 34-42. https://doi.org/10.1016/j.foodchem.2015.06.087
Kumar, K. K., Singh, S., Chakraborthy, S., Das, J., Bajaj, M., Hemanth, V., ... & Banerjee, P. (2019). Recycling fish skin for utilization in food industry as an effective emulsifier and foam stabilizing agent. Turkish Journal of Biochemistry, 44(3), 332-343.
Kurniasih, M., & Kartika, D. (2011). Sintesis dan karakterisasi fisika-kimia kitosan. Jurnal inovasi, 5(1), 42-48.
Lachman, L., A.H. Lieberman & J.L. Kanig. (2008). Teori dan Praktek Farmasi Industri, Edisi III. UI Press, Jakarta.
Lehninger, A. L. (1982). Proton and electric charge translocation in mitochondrial energy transduction. In Structure and Function Relationships in Biochemical Systems (pp. 171-186). Boston, MA: Springer US.
León-López, A., Morales-Peñaloza, A., Martínez-Juárez, V. M., Vargas-Torres, A., Zeugolis, D. I., & Aguirre-Álvarez, G. (2019). Hydrolyzed collagen—sources and applications. Molecules, 24(22), 4031.
Mahboob, S. (2015). Isolation and characterization of collagen from fish waste material-skin, scales and fins of Catla catla and Cirrhinus mrigala. Journal of food science and technology, 52(7), 4296-4305.
Manikkam, V., Mathai, M. L., Street, W. A., Donkor, O. N., & Vasiljevic, T. (2016). Biofunctional and physicochemical properties of fish scales collagen-derived protein powders. International Food Research Journal, 23(4), 1614.
Martin, H. (1993). The mechanisms of petrogenesis of the Archaean continental crust—comparison with modern processes. Lithos, 30(3-4), 373-388. https://doi.org/10.1016/0024-4937(93)90046-F
Massod, D., Ganai, A. M., Sheikh, G. G., Farooq, J., Afzal, Y., & Ahmad, I. (2018). Effect of feeding graded levels of apple pomace on growth performance, haemato-biochemical parameters and rumen metabolites in crossbred calves. Indian J Anim Res, 52, 1000-4.
Maulana, A., Adiandri, R. S., Boopathy, R., & Setiadi, T. (2022). Extraction, characterization, and biosurfactant properties of extracellular polymeric substance from textile wastewater activated sludge. Journal of bioscience and bioengineering, 134(6), 508-512. https://doi.org/10.1016/j.jbiosc.2022.08.005
Noomhorm, A., Ahmad, I., & Anal, A. K. (Eds.). (2014). Functional foods and dietary supplements: processing effects and health benefits. John Wiley & Sons.
Norman, W. (1988). Teknologi Pengawetan Pangan. Jakarta : UI Press.
Nurhidayah, B., Soekendars, E., & Erviani, A. E. (2019). Kandungan kolagen sisik ikan bandeng Chanos-chanos dan sisik ikan nila Oreochromis niloticus. BIOMA: Jurnal Biologi Makassar, 4(1), 39-47.
Pamungkas, B. F., Supriyadi, S., Murdiati, A., & Indrati, R. (2018). Ekstraksi dan karakterisasi kolagen larut asam dan pepsin dari sisik haruan (Channa striatus) kering. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(3), 513-521.
Paudi, R., Sulistijowati, R., & Mile, L. (2020). Rendemen kolagen kulit ikan bandeng (Chanos chanos) segar hasil ekstraksi asam asetat. Jambura Fish Processing Journal, 2 (1), 21–27.
Poedjiadi, A., & Supriyanti, F. M. T. (2005). Dasar-dasar biokimia.
Regenstein, J. M., & Zhou, P. (2007). Collagen and gelatin from marine by-products. In Maximising the value of marine by-products (pp. 279-303). Woodhead Publishing. https://doi.org/10.1533/9781845692087.2.279
Rocha, R., Aziz, S. A., Brook, C. E., Carvalho, W. D., Cooper-Bohannon, R., Frick, W. F., ... & Peel, A. J. (2020). Bat conservation and zoonotic disease risk: a research agenda to prevent misguided persecution in the aftermath of COVID-19. Animal Conservation.
Romadhon, R., Darmanto, Y. S., & Kurniasih, R. A. (2019). Karakteristik kolagen dari tulang, kulit, dan sisik ikan nila. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(2), 403-410.
Saari, H., Heravifar, K., Rayner, M., Wahlgren, M., & Sjöö, M. (2016). Preparation and characterization of starch particles for use in pickering emulsions. Cereal Chemistry, 93(2), 116-124.
de Castro Santana, R., Sato, A. C. K., & Da Cunha, R. L. (2012). Emulsions stabilized by heat-treated collagen fibers. Food hydrocolloids, 26(1), 73-81. https://doi.org/10.1016/j.foodhyd.2011.04.006
Shon, Z. H., Kim, K. H., & Song, S. K. (2011). Long-term trend in NO2 and NOx levels and their emission ratio in relation to road traffic activities in East Asia. Atmospheric Environment, 45(18), 3120-3131. https://doi.org/10.1016/j.atmosenv.2011.03.009
Sibilla, S., Godfrey, M., Brewer, S., Budh-Raja, A., & Genovese, L. (2015). An overview of the beneficial effects of hydrolysed collagen as a nutraceutical on skin properties: Scientific background and clinical studies. Open Nutraceuticals J, 8(1), 29-42.
Sun, C., & Gunasekaran, S. (2009). Effects of protein concentration and oil-phase volume fraction on the stability and rheology of menhaden oil-in-water emulsions stabilized by whey protein isolate with xanthan gum. Food Hydrocolloids, 23(1), 165-174. https://doi.org/10.1016/j.foodhyd.2007.12.006
Suptijah, P., Indriani, D., & Wardoyo, S. E. (2018). Isolasi dan karakterisasi kolagen dari kulit ikan patin (Pangasius sp.). Sains Natural: Journal of Biology and Chemistry, 8(1), 8-23.
Suryani, R. Hamsidi & N. Ikawati. (2015). Uji Stabilitas dan Batas Lapisan Emulsi. Prosiding Seminar Nasional Swasembada Pangan, 2, 234-241.
Toha, A., Zulkarnain, I., & Purnamasari, V. (2020). Extract As Antihyperpigmentation With Various Concentration Of Emulgators. Jurnal Ilmiah Farmako Bahari Aztriana Toha, 11(1).
Veeruraj, A., Arumugam, M., & Balasubramanian, T. (2013). Isolation and characterization of thermostable collagen from the marine eel-fish (Evenchelys macrura). Process biochemistry, 48(10), 1592-1602. https://doi.org/10.1016/j.procbio.2013.07.011
Voigt, R. (1995). Buku Pelajaran Teknologi Farmasi, Edisi V. Yogyakarta: Gadjah Mada Universiti Press
Wahyu, I.Y. (2018). Optimasi Proses Pretreatment Pada Sisik Ikan Bandeng (chanos chanos frskal) Dengan Response Surface Methodology. Prosiding Seminar Nasional Kelautan dan Perikanan IV.
Wang, J., Duncan, D., Shi, Z., & Zhang, B. (2013). WEB-based gene set analysis toolkit (WebGestalt): update 2013. Nucleic acids research, 41(W1), W77-W83.
Widria, Y., Trilaksani, W., & Cahyadi, E. R. (2016). Evaluasi dan pengembangan sistem manajemen rantai pasok bandeng segar (Chanos chanos) di Kota Bekasi, Jawa Barat. MANAJEMEN IKM: Jurnal Manajemen Pengembangan Industri Kecil Menengah, 11(2), 129-140.
Zdzieblik, D., Oesser, S., Gollhofer, A., & König, D. (2017). Improvement of activity-related knee joint discomfort following supplementation of specific collagen peptides. Applied Physiology, Nutrition, and Metabolism, 42(6), 588-595.
Published
How to Cite
Issue
Section
Copyright (c) 2023 International journal of health & medical sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health & Medical Sciences (IJHMS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHMS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHMS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.


